Fig. 2

Intermittent FMD reduced hepatic steatosis in db/db mice. a Representative H&E staining of liver sections. Scale bar: 50 μm. b Levels of total triglycerides and total cholesterol in the liver. c Blood ALT and AST levels of the mice. d Quantitative RT-PCR results to detect expression of genes involved in peroxisomal fatty acid oxidation, lipogenesis, lipid secretion, lipid uptake, and inflammation. Mouse livers were used to isolate total RNA, followed by reverse transcription and real-time PCR. Data are expressed as the mean ± SEM, n = 8 per group. * p < 0.05, *** p < 0.001, ns for nonsignificant