Fig. 3

Intermittent FMD restores the cells of pancreatic islets in db/db mice. a Representative H&E staining of islets in pancreatic sections. Scale bar: 50 μm. The ratio of islet area in the pancreas sections is shown in the right panel. b Immunostaining of pancreatic sections to detect expression of insulin and glucagon. Scale bar: 20 μm. c Percentage of β cells and α cells per islet. d Serum insulin levels under fasting and fed state. Blood samples were collected at week 9. Mice were fasted for 6 h for blood glucose measurements or without fasting. Data are expressed as the mean ± SEM, n = 8 per group. *p < 0.05, *** < 0.001